Overview

  • Product name

  • Description

    Guinea pig polyclonal to Substance P
  • Host species

    Guinea pig
  • Tested applications

    Suitable for: IHC-FoFr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Cynomolgus monkey
  • Immunogen

    Synthetic peptide: NH2-

    CRPKPQQFFGLM

    -CONH2, corresponding to amino acids 1-11 of Rat Substance P.

  • Positive control

    • Rat spinal cord

Properties

Applications

Our Abpromise guarantee covers the use of ab106291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use a concentration of 15 µg/ml. Perfusion: 1) calcium free Tyrodes solution, 2) paraformaldehyde picric acid fixative, and 3) 10% sucrose in PBS as a cryo protectant.
ICC/IF Use at an assay dependent dilution.

Target

Images

  • ab106291, at 15 µg/ml, staining Substance P in Rat spinal cord dorsal horn (red fluorescence) by Immunofluorescence.
  • ab106291, at 15 µg/ml, staining Substance P in Rat DRG (red fluorescence) by Immunofluorescence.

References

This product has been referenced in:

  • Hu S  et al. Antifertility effectiveness of a novel copper-containing intrauterine device material and its influence on the endometrial environment in rats. Mater Sci Eng C Mater Biol Appl 89:444-455 (2018). Read more (PubMed: 29752117) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.


I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species.

Here are the results, similarities are in %:

ab47027 Anti-CGRP antibody: 85%

ab14184 Anti-Substance P antibody [SP-DE4-21]: 100%

ab106291 Anti-Substance P antibody: 100%

ab2318: As mentioned in the Scientific Support section, PGE2 is a lipid rather than a protein and is therefore not species specific. There should not be any particular problems using this assay to detect PGE2 from rabbit. Optimization for different sample types will have to be done by the end user. Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.


I can offer a discount off a future purchase if your customer buys one or more of the antibodiesnow, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.


If they are interested in this offer, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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