Product nameAnti-Substance P antibody [EPR3959]
See all Substance P primary antibodies
DescriptionRabbit monoclonal [EPR3959] to Substance P
Tested applicationsSuitable for: IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human Substance P aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P20366
- Human brain tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified, use 1/50 - 1/100.
FunctionTachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Sequence similaritiesBelongs to the tachykinin family.
- Information by UniProt
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab133240 at a working dilution of 1 in 250. The secondary antibody used is a HRP goat anti-rabbit (ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemical analysis of Substance P in paraffin embedded human brain tissue, using unpurified ab133240 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab133240 has been referenced in 1 publication.
- Nishijima H et al. Denervation of nasal mucosa induced by posterior nasal neurectomy suppresses nasal secretion, not hypersensitivity, in an allergic rhinitis rat model. Lab Invest 96:981-93 (2016). PubMed: 27322954