Anti-Substance P antibody [SP-DE4-21] (ab14184)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-240737-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Key features and details
- Mouse monoclonal [SP-DE4-21] to Substance P
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Guinea pig, Human
- Isotype: IgG1
Overview
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Product name
Anti-Substance P antibody [SP-DE4-21]
See all Substance P primary antibodies -
Description
Mouse monoclonal [SP-DE4-21] to Substance P -
Host species
Mouse -
Specificity
The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin). -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Guinea pig, Human
Predicted to work with: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
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Immunogen
Synthetic peptide corresponding to Human Substance P aa 58-68.
Sequence:RPKPQQFFGLM
Database link: P20366 -
Positive control
- Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.75% Glycine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SP-DE4-21 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/2000. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. -
Sequence similarities
Belongs to the tachykinin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 281512 Cow
- Entrez Gene: 6863 Human
- Entrez Gene: 24806 Rat
- Omim: 162320 Human
- SwissProt: P19850 Chicken
- SwissProt: P01289 Cow
- SwissProt: P67932 Guinea pig
- SwissProt: Q60541 Hamster
see all -
Alternative names
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbs
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21716-anti-substance-p-antibody-sp-de4-21-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21715-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21717-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
References (26)
ab14184 has been referenced in 26 publications.
- Evdokimov D et al. Characterization of dermal skin innervation in fibromyalgia syndrome. PLoS One 15:e0227674 (2020). PubMed: 31929578
- Zhang Y et al. Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points. Evid Based Complement Alternat Med 2020:5129562 (2020). PubMed: 32190087
- Blumer R et al. How to visualize the innervation pattern in tendons: A methodical guide. Ann Anat 225:21-27 (2019). PubMed: 31195096
- Aoki S et al. An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway. Elife 8:N/A (2019). PubMed: 31490123
- Sorby-Adams AJ et al. NK1-r Antagonist Treatment Comparable to Decompressive Craniectomy in Reducing Intracranial Pressure Following Stroke. Front Neurosci 13:681 (2019). PubMed: 31333402
