Key features and details
- Mouse monoclonal [SP-DE4-21] to Substance P
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Guinea pig, Human
- Isotype: IgG1
Product nameAnti-Substance P antibody [SP-DE4-21]
See all Substance P primary antibodies
DescriptionMouse monoclonal [SP-DE4-21] to Substance P
SpecificityThe antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Guinea pig, Human
Predicted to work with: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
- Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Constituents: PBS, 0.75% Glycine
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab14184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionTachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Sequence similaritiesBelongs to the tachykinin family.
- Information by UniProt
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.
Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
ab14184 has been referenced in 26 publications.
- Evdokimov D et al. Characterization of dermal skin innervation in fibromyalgia syndrome. PLoS One 15:e0227674 (2020). PubMed: 31929578
- Zhang Y et al. Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points. Evid Based Complement Alternat Med 2020:5129562 (2020). PubMed: 32190087
- Blumer R et al. How to visualize the innervation pattern in tendons: A methodical guide. Ann Anat 225:21-27 (2019). PubMed: 31195096
- Aoki S et al. An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway. Elife 8:N/A (2019). PubMed: 31490123
- Sorby-Adams AJ et al. NK1-r Antagonist Treatment Comparable to Decompressive Craniectomy in Reducing Intracranial Pressure Following Stroke. Front Neurosci 13:681 (2019). PubMed: 31333402