Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Dog Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Strong punctate labelling of fibres in Meissner's plexus ( not shown and also in Auerbach's plexus ( black arrowhead).
A similar positivity is seen in fibres in the muscle layer ( green arrowheads).
Rbcs ( red arrowhead) and cytoplasm in general give a slight positivity that I cannot get rid of, without losing intensity of specific staining.
In practice , this does not worry me.
MR. Carl Hobbs
Verified customer
Submitted Nov 18 2014