Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain and jejunum)
Specification
Brain and jejunum
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted composite image indicates that ab14184 is not as good as ab10353 in rat brain ( upper image).
This is supported by lower image of rat jejunum where the specific positivity is similar to that obtained using ab10353 but, there is more non-specific "background" with this antibody.
However, a useful reagent where more than single labelling is required.
Arrowheads: green for positive nerve fibres in muscularis interna and lamina propria red for positive cells amongst enterocytes black for positive ganglion fibres of Auerbach's plexus.
Mr. Carl Hobbs
Verified customer
Submitted Feb 13 2015