Overview

  • Product name

    Substance P ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc 24 101pg/ml 6.7%
    Medium conc 24 1116pg/ml 4.5%
    High conc 24 6257pg/ml 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Low conc 8 97pg/ml 4.2%
    Medium conc 8 1120pg/ml 7.3%
    High conc 8 6402pg/ml 7.3%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    8.04 pg/ml
  • Range

    9.8 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 108.3 % - %
    Urine 105.8 % - %
    Serum 97.7 % - %
    Tissue Culture Media 81 % - %
    Heparin Plasma 109.3 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Substance P in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Substance P in biological fluids.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Substance P captured in the plate.

  • Notes

    Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman’s group in 1970. The structure is shown below.

    Substance P:  H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2

    Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.

    Cross Reactivity

    Compound% Cross Reactivity
    Substance P100
    Substance P (3-11)

    85.9

    Physalaemin75.3
    Substance P (4-11)11.7
    Substance P (7-11)5.9
    α-Neurokinin0.8
    β-Neurokinin0.2
    Somatostatin<0.001
    Substance P (1-4)<0.001
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Substance P measured in human, rat and pig biological fluids; background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab133029.

Protocols

References

This product has been referenced in:

  • Davoodian M  et al. Evaluation of serum level of substance P and tissue distribution of NK-1 receptor in breast cancer. Mol Biol Rep 46:1285-1293 (2019). Read more (PubMed: 30684188) »
  • Rahman MM  et al. Chondroprotective Effects of a Standardized Extract (KBH-JP-040) from Kalopanax pictus, Hericium erinaceus, and Astragalus membranaceus in Experimentally Induced In Vitro and In Vivo Osteoarthritis Models. Nutrients 10:N/A (2018). Read more (PubMed: 29543781) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Substance P is species independent and will work with any species as long as there is enough Substance P for the kit to reliably detect. Rabbit samples containing endogenous IgG will likely cause interference given that the kit comes with goat anti-rabbit IgG plates. Outside of that sample consideration, the actual molecule itself, Substance P, is conserved between species so yes, rat should work if the samples have levels above the lower limit of detection after sample handling/dilution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

We have not tested for reactivity with hemokinin-1, so unfortunately we do not have any data in this regard.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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