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I was curious whether this ELISA (ab133029 ) will cross-react with Substance P from rat?
Asked on Feb 14 2013
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Substance P is species independent and will work with any species as long as there is enough Substance P for the kit to reliably detect. Rabbit samples containing endogenous IgG will likely cause interference given that the kit comes with goat anti-rabbit IgG plates. Outside of that sample consideration, the actual molecule itself, Substance P, is conserved between species so yes, rat should work if the samples have levels above the lower limit of detection after sample handling/dilution.
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Answered on Feb 14 2013