Key features and details
- Fluorogenic substrate of calpains and 20S proteasome
- CAS Number: 94367-21-2
- Purity: > 97%
- Soluble in DMSO to 25 mM
- Form / State: Solid
- Source: Synthetic
Product nameSuc-Leu-Leu-Val-Tyr-AMC, Fluorogenic substrate of calpains and 20S proteasome
DescriptionFluorogenic substrate of calpains and 20S proteasome
Fluorogenic substrate of calpains and 20S proteasome. Calpain I and II hydrolyses Suc-Leu-Leu-Val-Tyr-AMC with Km values of 203 and 466 µM, respectively. Used to study the activity of calpains and proteosomal degradation.
SequenceLLVY (Modifications: N-terminal succinyl; C-terminal AMC)
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab142120 has been referenced in 3 publications.
- Liu X et al. Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation. Apoptosis 26:195-208 (2021). PubMed: 33550458
- Voss TD et al. Ubiquitination and the proteasome rather than caspase-3-mediated C-terminal cleavage are involved in the EAAT2 degradation by staurosporine-induced cellular stress. J Neurochem 157:1284-1299 (2021). PubMed: 33180957
- Zhang L et al. A Bowman-Birk type chymotrypsin inhibitor peptide from the amphibian, Hylarana erythraea. Sci Rep 8:5851 (2018). PubMed: 29643444