Overview

  • Product name

    Succinate Dehydrogenase Activity Assay Kit (Colorimetric)
    See all Complex II kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture extracts, Tissue Homogenate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    < 0.1 mU/well
  • Product overview

    Succinate Dehydrogenase Activity Assay Kit (Colorimetric) (ab228560) is rapid, simple and high-throughput adaptable. In this assay, Succinate Dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity). This assay kit can detect less than 0.1mU Succinate Dehydrogenase activity in a variety of samples. 


    The kit provides enough reagents for 100 assays using the methods as described.                                                                           

  • Notes

    Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    DCIP Standard (2 mM) 1 x 0.4ml
    SDH Assay Buffer 1 x 25ml
    SDH Positive Control (Lyophilized) 1 vial
    SDH Probe 1 x 0.2ml
    SDH Substrate Mix (lyophilized) 1 vial
  • Research areas

  • Relevance

    Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
  • Alternative names

    • SDH
    • Succinate coenzyme Q reductase
    • Succinate dehydrogenase

Images

  • (a) DCIP standard curve, (b) typical assay data and (c) succinate dehydrogenase activity in mitochondria isolated from mouse heart (24 μg) and liver (70 μg).

Protocols

References

ab228560 has not yet been referenced specifically in any publications.

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