Product nameAnti-SUCLA2 antibody
See all SUCLA2 primary antibodies
DescriptionRabbit polyclonal to SUCLA2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human SUCLA2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q9P2R7
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Mitochondrial Heart ; Mouse Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heartmuscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/250. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).|
FunctionCatalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
Tissue specificityWidely expressed. Not expressed in liver and lung.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Involvement in diseaseMitochondrial DNA depletion syndrome 5
Sequence similaritiesBelongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain.
- Information by UniProt
- A BETA antibody
- A SCS antibody
- ATP specific succinyl CoA synthetase subunit beta antibody
IHC image of SUCLA2 staining in Human normal heartmuscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab133956, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-SUCLA2 antibody (ab133956) at 1/250 dilution
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa (possible non-specific binding), 58 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)
Exposure time: 4 minutes
The band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133956 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab133956 has not yet been referenced specifically in any publications.