Overview

  • Product name

  • Description

    Rabbit polyclonal to SUCLA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SUCLA2 aa 200-300 conjugated to keyhole limpet haemocyanin.
    Database link: Q9P2R7

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Mitochondrial Heart ; Mouse Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heartmuscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab133956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
  • Tissue specificity

    Widely expressed. Not expressed in liver and lung.
  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
  • Involvement in disease

    Mitochondrial DNA depletion syndrome 5
  • Sequence similarities

    Belongs to the succinate/malate CoA ligase beta subunit family.
    Contains 1 ATP-grasp domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • A BETA antibody
    • A SCS antibody
    • ATP specific succinyl CoA synthetase subunit beta antibody
    • ATP specific succinyl CoA synthetase, beta subunit antibody
    • ATP-specific succinyl-CoA synthetase subunit beta antibody
    • Mitochondrial succinyl CoA ligase [ADP forming] subunit beta antibody
    • MTDPS5 antibody
    • Renal carcinoma antigen NY-REN-39 antibody
    • Renal carcinoma antigen NYREN39 antibody
    • SCS betaA antibody
    • SCS-betaA antibody
    • SUCB1_HUMAN antibody
    • Succinate CoA ligase (ADP forming) antibody
    • Succinate CoA ligase [ADP forming] subunit beta, mitochondrial; succinyl CoA ligase [ADP forming] subunit beta, mitochondrial antibody
    • Succinate CoA ligase ADP forming beta subunit antibody
    • Succinate CoA ligase beta subunit antibody
    • Succinyl CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl CoA synthetase beta A chain antibody
    • Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl-CoA synthetase beta-A chain antibody
    • SUCLA2 antibody
    see all

Images

  • IHC image of SUCLA2 staining in Human normal heartmuscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab133956, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-SUCLA2 antibody (ab133956) at 1/250 dilution

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human Heart Mitochondrial Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 17 kDa (possible non-specific binding), 58 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)


    Exposure time: 4 minutes


    The band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133956 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab133956 has not yet been referenced specifically in any publications.

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