Key features and details
- Rabbit polyclonal to SUCLA2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SUCLA2 antibody
See all SUCLA2 primary antibodies
DescriptionRabbit polyclonal to SUCLA2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Human SUCLA2 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9P2R7
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Mitochondrial Heart ; Mouse Heart; Mouse Liver as well as both HepG2 and MOLT4 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).|
FunctionCatalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
Tissue specificityWidely expressed. Not expressed in liver and lung.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Involvement in diseaseMitochondrial DNA depletion syndrome 5
Sequence similaritiesBelongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain.
- Information by UniProt
- A BETA antibody
- A SCS antibody
- ATP specific succinyl CoA synthetase subunit beta antibody
All lanes : Anti-SUCLA2 antibody (ab190845) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 30 seconds
The band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab190845 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab190845 has not yet been referenced specifically in any publications.