Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14925] to SUCLA2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-SUCLA2 antibody [EPR14925]
See all SUCLA2 primary antibodies
DescriptionRabbit monoclonal [EPR14925] to SUCLA2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SUCLA2 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q9P2R7
- Human fetal liver, 293, HepG2 and Molt4 cell lysates, Human breast tissue, HepG2 and 293 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/40 - 1/60.|
FunctionCatalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
Tissue specificityWidely expressed. Not expressed in liver and lung.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Involvement in diseaseMitochondrial DNA depletion syndrome 5
Sequence similaritiesBelongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain.
- Information by UniProt
- A BETA antibody
- A SCS antibody
- ATP specific succinyl CoA synthetase subunit beta antibody
All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt4 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.
Flow cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab183513 has not yet been referenced specifically in any publications.