Key features and details
- Rabbit polyclonal to SUCLG2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SUCLG2 antibody
See all SUCLG2 primary antibodies
DescriptionRabbit polyclonal to SUCLG2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig
Synthetic peptide within Human SUCLG2 aa 25-75. The exact sequence is proprietary.
Database link: Q96I99
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241277 was affinity purified using an epitope specific to SUCLG2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 47 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCatalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA.
Tissue specificityMainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle.
Sequence similaritiesBelongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain.
- Information by UniProt
- EC 188.8.131.52 antibody
- G BETA antibody
- GBETA antibody
All lanes : Anti-SUCLG2 antibody (ab241277) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
Lysates prepared in NETN buffer.
SUCLG2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241277 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241277 at 1 µg/ml.
Lane 1: ab241277 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241277 has not yet been referenced specifically in any publications.