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Signal Transduction Metabolism Energy Metabolism
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Anti-SUCLG2 antibody (ab96172)

  • Datasheet
  • SDS
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Western blot - Anti-SUCLG2 antibody (ab96172)

    Key features and details

    • Rabbit polyclonal to SUCLG2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-SUCLG2 antibody
      See all SUCLG2 primary antibodies
    • Description

      Rabbit polyclonal to SUCLG2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow
    • Immunogen

      Recombinant fragment, corresponding to a region within the N terminal amino acids 1-204 of Human SUCLG2.

    • Positive control

      • 293T, A431, H1299, HeLa S3, HepG2, MOLT4 or Raji cell lysates
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Raji whole cell lysate (ab30124)
      • Raji membrane extract lysate (ab30126)
      • A-431 membrane extract lysate (ab30134)
      • HEK-293 whole cell lysate (ab7902)
      • A-431 whole cell lysate (ab7909)
      • MOLT-4 whole cell lysate (ab7912)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96172 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 47 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 47 kDa.

    Target

    • Function

      Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA.
    • Tissue specificity

      Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle.
    • Sequence similarities

      Belongs to the succinate/malate CoA ligase beta subunit family.
      Contains 1 ATP-grasp domain.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q96I99 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 537713 Cow
      • Entrez Gene: 8801 Human
      • Entrez Gene: 20917 Mouse
      • Entrez Gene: 362404 Rat
      • Omim: 603922 Human
      • SwissProt: Q3MHX5 Cow
      • SwissProt: Q96I99 Human
      • SwissProt: Q9Z2I8 Mouse
      • Unigene: 644919 Human
      • Unigene: 371585 Mouse
      see all
    • Alternative names

      • EC 6.2.1.4 antibody
      • G BETA antibody
      • GBETA antibody
      • GTP specific succinyl-CoA synthetase beta subunit antibody
      • GTP specific succinyl-CoA synthetase subunit beta antibody
      • GTP-specific succinyl-CoA synthetase subunit beta antibody
      • mitochondrial antibody
      • SCS betaG antibody
      • SCS-betaG antibody
      • SUCB2_HUMAN antibody
      • Succinate CoA ligase GDP forming beta subunit antibody
      • Succinate-Coenzyme A ligase, GDP-forming, beta subunit antibody
      • Succinyl CoA ligase GDP forming beta chain mitochondrial antibody
      • Succinyl CoA synthetase betaG chain antibody
      • Succinyl-CoA ligase [GDP-forming] subunit beta antibody
      • Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial antibody
      • Succinyl-CoA synthetase beta-G chain antibody
      • SUCLG2 antibody
      see all

    Images

    • Western blot - Anti-SUCLG2 antibody (ab96172)
      Western blot - Anti-SUCLG2 antibody (ab96172)
      All lanes : Anti-SUCLG2 antibody (ab96172) at 1/1000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : H1299 whole cell lysate
      Lane 4 : HeLa S3 whole cell lysate
      Lane 5 : Hep G2 whole cell lysate
      Lane 6 : MOLT4 whole cell lysate
      Lane 7 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 47 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab96172? Please let us know so that we can cite the reference in this datasheet.

    ab96172 has been referenced in 2 publications.

    • Zhang W  et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856
    • Dobolyi A  et al. Exclusive neuronal expression of SUCLA2 in the human brain. Brain Struct Funct 220:135-51 (2015). PubMed: 24085565

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