Sudan black B, Fat-soluble diazo dye (ab146284)

Overview

  • Product name

    Sudan black B, Fat-soluble diazo dye
  • Description

    Fat-soluble diazo dye.
  • Alternative names

    • 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-pyrimidine
    • CI 26150
    • Fat Black HB
    • Solvent Black 3
    • Solvent Black B1
    see all
  • Biological description

    Fat-soluble diazo dye primarily used for staining of neutral triglycerides and lipids. Sudan dyes are a group of lipid soluble dyes often called lysochromes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Useful for detecting fats that are contaminated with oil and grease. Used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts.

  • CAS Number

    4197-25-5
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-pyrimidine
  • Molecular weight

    456.55
  • Molecular formula

    C29H24N6
  • Storage instructions

    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Zorn U  et al. Characterization of modified low density lipoprotein subfractions by capillary isotachophoresis. Electrophoresis 22:1143-9 (2001). Read more (PubMed: 11358140) »
  • Baschong W  et al. Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J Histochem Cytochem 49:1565-72 (2001). Read more (PubMed: 11724904) »
  • Schnell SA  et al. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem 47:719-30 (1999). Read more (PubMed: 10330448) »
  • Frederiks WM  et al. (Histo)chemical aspects of Sudan Black B in various solvents. Acta Histochem Suppl 24:259-65 (1981). Read more (PubMed: 6785833) »

Customer reviews and Q&As

Answer


Sudan Black B (ab146284) is soluble in ethanol (10 mg/ml), yielding a dark blue solution. This may require heat for complete solubilization. It is also soluble in acetone, benzene, toluene, hydrocarbon solvents, fats, oils, and paraffins. It is slightly soluble in water (0.1 mg/ml).

The choice of the solvent depends on the downstream application. For example, to stain lipoprotein after polyacrylamide gel electrophoresis, a staining solution of 500 mg Sudan Black B is prepared in 20 ml of acetone. This is then added to 15 ml of acetic acid, and then added to 85 ml of water.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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