Overview

  • Product name

  • Description

    Rabbit polyclonal to SUFU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SUFU aa 434-484. The exact sequence is proprietary. NP_057253.2
    Database link: Q9UMX1

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: SUFU IP in HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000.

Target

  • Function

    Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24311597, PubMed:24217340). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation.
  • Tissue specificity

    Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.
  • Involvement in disease

    Medulloblastoma
  • Sequence similarities

    Belongs to the SUFU family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • OTTHUMP00000020374 antibody
    • OTTHUMP00000020377 antibody
    • OTTHUMP00000020379 antibody
    • PRO1280 antibody
    • SU FU antibody
    • SU(F)U antibody
    • Su(fu) antibody
    • SUFU antibody
    • SUFU negative regulator of hedgehog signaling antibody
    • SUFU_HUMAN antibody
    • SUFUH antibody
    • SUFUXL antibody
    • Suppressor of Fused antibody
    • Suppressor of fused homolog (Drosophila) antibody
    • Suppressor of fused homolog antibody
    see all

Images

  • All lanes : Anti-SUFU antibody (ab245428) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Exposure time: 30 seconds
  • SUFU was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245428 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245428 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 10 seconds.

References

ab245428 has not yet been referenced specifically in any publications.

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