• Product name

  • Description

    Rabbit polyclonal to SUFU
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SUFU aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab28932, ab28933)

  • Positive control

    • Recombinant Human SUFU protein (ab113584) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) This antibody gave a positive signal in the following tissue lysate: Testis (Mouse)



Our Abpromise guarantee covers the use of ab28083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).


  • Function

    Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24311597, PubMed:24217340). Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340). Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10806483, PubMed:24217340). Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation.
  • Tissue specificity

    Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.
  • Involvement in disease

  • Sequence similarities

    Belongs to the SUFU family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • OTTHUMP00000020374 antibody
    • OTTHUMP00000020377 antibody
    • OTTHUMP00000020379 antibody
    • PRO1280 antibody
    • SU FU antibody
    • SU(F)U antibody
    • Su(fu) antibody
    • SUFU antibody
    • SUFU negative regulator of hedgehog signaling antibody
    • SUFU_HUMAN antibody
    • SUFUH antibody
    • SUFUXL antibody
    • Suppressor of Fused antibody
    • Suppressor of fused homolog (Drosophila) antibody
    • Suppressor of fused homolog antibody
    see all


  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SUFU knockout HAP1 cell lysate (20 µg)
    Lane 3: LnCaP cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 to 4: Merged signal (red and green). Green - ab28083 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab28083 was shown to recognize SUFU when SUFU knockout samples were used, along with additional cross-reactive bands. Wild-type and SUFU knockout samples were subjected to SDS-PAGE. ab28083 and ab8245 (loading control to GAPDH) were both diluted at 1 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-SUFU antibody (ab28083) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa
    Additional bands at: 37 kDa (possible cleavage fragment), 37 kDa (possible cross reactivity)

  • Anti-SUFU antibody (ab28083) at 1 µg/ml + Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • ICC/IF image of ab28083 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28083, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).


This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Mouse Tissue lysate - whole (brain)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 12 2012

Western blot
Human Cell lysate - whole cell (HEK293, SKOV-3, CaOV-3)
Loading amount
15 µg
HEK293, SKOV-3, CaOV-3
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Aqua Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 07 2011


Thank you for your enquiry. This antibody has only been tested in Western Blot. That does not mean that it won't work in IHC applications, it just means that we haven't tested it and therefore can not guarantee that it will work in those applications. If you choose to purchase the antibody and use it for IHC, I encourage you to let us know how it goes by submitting an Abreview. By submitting Abreviews, you can earn Abpoints which can be used towards various promotions such as Amazon.com gift certificates or a future antibody purchase. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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