Product nameAnti-SUGT1 antibody
See all SUGT1 primary antibodies
DescriptionRabbit polyclonal to SUGT1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Chimpanzee, Gorilla, Common marmoset, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Sequence similaritiesBelongs to the SGT1 family.
Contains 1 CS domain.
Contains 1 SGS domain.
Contains 3 TPR repeats.
DomainThe CS domain mediates interaction with HSP90.
modificationsPhosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
- Information by UniProt
- Protein 40 6 3 antibody
- Protein 40-6-3 antibody
- Protein SGT1 homolog antibody
All lanes : Anti-SUGT1 antibody (ab176849) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
Detection of SUGT1 by Western Blot of Immunoprecipitate.
ab176849 at 0.4 µg/ml labeling SUGT1 in HeLa whole cell lysate immunoprecipitated using ab176849 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds
ab176849 has not yet been referenced specifically in any publications.