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    sugt1-antibody-ab204428.pdf

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Epigenetics and Nuclear Signaling Cell cycle Chromosome Structure Chromatid Cohesion
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Anti-SUGT1 antibody (ab204428)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUGT1 antibody (ab204428)
  • Western blot - Anti-SUGT1 antibody (ab204428)
  • Immunocytochemistry/ Immunofluorescence - Anti-SUGT1 antibody (ab204428)

Key features and details

  • Rabbit polyclonal to SUGT1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SUGT1 protein (ab103785)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SUGT1 antibody
  • Description

    Rabbit polyclonal to SUGT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human SUGT1 aa 297-363.
    Sequence:

    EEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSD VGKRKVEINPPDDMEWK


    Database link: Q9Y2Z0
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • RT-4, U-251 MG, Human plasma and Human liver lysates; Human testis tissue; A431 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SUGT1 protein (ab103785)

Applications

Our Abpromise guarantee covers the use of ab204428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • Function

    May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
  • Sequence similarities

    Belongs to the SGT1 family.
    Contains 1 CS domain.
    Contains 1 SGS domain.
    Contains 3 TPR repeats.
  • Domain

    The CS domain mediates interaction with HSP90.
  • Post-translational
    modifications

    Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
  • Target information above from: UniProt accession Q9Y2Z0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515509 Cow
    • Entrez Gene: 10910 Human
    • Entrez Gene: 67955 Mouse
    • Entrez Gene: 290408 Rat
    • Omim: 604098 Human
    • SwissProt: Q2KIK0 Cow
    • SwissProt: Q9Y2Z0 Human
    • SwissProt: Q9CX34 Mouse
    • SwissProt: B0BN85 Rat
    • Unigene: 281902 Human
    • Unigene: 18972 Mouse
    • Unigene: 101758 Rat
    see all
  • Alternative names

    • Protein 40 6 3 antibody
    • Protein 40-6-3 antibody
    • Protein SGT1 homolog antibody
    • Putative 40 6 3 protein antibody
    • Sgt1 antibody
    • SGT1 suppressor of G2 allele of SKP1 antibody
    • SGT1_HUMAN antibody
    • SGT1B protein antibody
    • sugt1 antibody
    • Suppressor of G2 allele of SKP1 homolog antibody
    • Suppressor of G2 allele of SKP1 S. cerevisiae homolog of antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUGT1 antibody (ab204428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUGT1 antibody (ab204428)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labeling SUGT1 with ab204428 at 1/50 dilution.

  • Western blot - Anti-SUGT1 antibody (ab204428)
    Western blot - Anti-SUGT1 antibody (ab204428)
    All lanes : Anti-SUGT1 antibody (ab204428) at 1/100 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SUGT1 antibody (ab204428)
    Immunocytochemistry/ Immunofluorescence - Anti-SUGT1 antibody (ab204428)

    Immunofluorescent analysis of formalin fixed, triton X-100 permealized A431 cells labeling SUGT1 with ab204428 at 4 µg/ml (green).

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab204428? Please let us know so that we can cite the reference in this datasheet.

    ab204428 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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