Key features and details
- Rabbit polyclonal to SUGT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SUGT1 antibody
DescriptionRabbit polyclonal to SUGT1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- RT-4, U-251 MG, Human plasma and Human liver lysates; Human testis tissue; A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
FunctionMay play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Sequence similaritiesBelongs to the SGT1 family.
Contains 1 CS domain.
Contains 1 SGS domain.
Contains 3 TPR repeats.
DomainThe CS domain mediates interaction with HSP90.
modificationsPhosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
- Information by UniProt
- Protein 40 6 3 antibody
- Protein 40-6-3 antibody
- Protein SGT1 homolog antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labeling SUGT1 with ab204428 at 1/50 dilution.
All lanes : Anti-SUGT1 antibody (ab204428) at 1/100 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Predicted band size: 41 kDa
Immunofluorescent analysis of formalin fixed, triton X-100 permealized A431 cells labeling SUGT1 with ab204428 at 4 µg/ml (green).
ab204428 has not yet been referenced specifically in any publications.