Product nameAnti-SUGT1 antibody
See all SUGT1 primary antibodies
DescriptionRabbit polyclonal to SUGT1
Specificityab30931 appears to detect both recorded isoforms of SGT1, at 41 kDa and 38 kDa.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- ab30931 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab30931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
FunctionMay play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Sequence similaritiesBelongs to the SGT1 family.
Contains 1 CS domain.
Contains 1 SGS domain.
Contains 3 TPR repeats.
DomainThe CS domain mediates interaction with HSP90.
modificationsPhosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus upon heat shock, requiring S100A6.
- Information by UniProt
- Protein 40 6 3 antibody
- Protein 40-6-3 antibody
- Protein SGT1 homolog antibody
All lanes : Anti-SUGT1 antibody (ab30931) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lane 4 :
HEK293 whole cell lysate (ab7902)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 38 kDa (possible isoform)
ICC/IF image of ab30931 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30931, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
- Gangula NR & Maddika S Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1. J Biol Chem 292:13947-13958 (2017). Read more (PubMed: 28696259) »
- Wang N et al. Comparative proteome profile of immature rat ovary during primordial follicle assembly and development. Proteomics 9:3425-34 (2009). Read more (PubMed: 19557761) »