Anti-Sulfatase 1/SULF1 antibody (ab168616)
Key features and details
- Mouse polyclonal to Sulfatase 1/SULF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sulfatase 1/SULF1 antibody
See all Sulfatase 1/SULF1 primary antibodies -
Description
Mouse polyclonal to Sulfatase 1/SULF1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein within Human Sulfatase 1/SULF1 aa 1-900. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_055985 -
Positive control
- Sulfatase 1/SULF1 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. |
Target
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Function
Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation. -
Tissue specificity
Expressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, breast, pancreatic, renal and hepatocellular carcinoma cell lines. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 23213 Human
- Entrez Gene: 240725 Mouse
- Entrez Gene: 171396 Rat
- Omim: 610012 Human
- SwissProt: Q8IWU6 Human
- SwissProt: Q8K007 Mouse
- SwissProt: Q8VI60 Rat
- Unigene: 409602 Human
see all -
Alternative names
- DSulf antibody
- Extracellular sulfatase Sulf 1 antibody
- Extracellular sulfatase Sulf-1 antibody
see all
Images
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All lanes : Anti-Sulfatase 1/SULF1 antibody (ab168616) at 1 µg/ml
Lane 1 : Sulfatase 1/SULF1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Datasheets and documents
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Datasheet download
References (0)
ab168616 has not yet been referenced specifically in any publications.