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One of our customers used AB32763 for western blotting and requires
technical support even though she followed Abcam protocol.
She was expecting the band size to be 132 kD but her actual results,
as seen in the image attached, was 170 kD.
The cell line that she uses:
Please advise on how we should proceed.
I look forward to your reply!
Asked on Apr 24 2012
Thank you for your email. I am sorry to hear that the customer is having trouble using this antibody.
The link you have provided does not show any information about the cell line so could you ask customer to fill the attached questionnaire.
Please also note that this is a fast track antibody. We do not have full characterization data for this antibody which is why it is much cheaper. The Abcam money back or replacement guarantee will not be applied to this product; the full terms and conditions are give on the datasheet. We are happy to provide technical help which may improve the product in customer hands however we will not be able to refund the product in case it does not work.
I hope this info will be helpful. I will look forward to receive the questionnaire soon.
Answered on Apr 24 2012