Question (45410) | Anti-Sulfatase 1/SULF1 antibody (ab32763)

Go to datasheet (ab32763)

Question

I have filled up thequestionnaireas attached.
However, I have left out the 1st line order details, please specify what it means.

I look forward to your response, the customer requires some urgent help.

Many Thanks and Best Regards,

Answer

Thank you for filling the questionnaire.

The protocol seems fine however there is only one suggestion we would like to offer; heating the lysates for 10 minutes at 100C may improve the results.

This protein is very heavily glycosylated so the observed band size will be higher than the predicted one. I would suggest searching literature for the observed band size.

Please also note that this is a fast track antibody. We do not have full characterization data for this antibody which is why it is much cheaper. The Abcam money back or replacement guarantee will not be applied to this product; the full terms and conditions are give on the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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