Western blot abreview for Anti-Sulfatase 1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (lung alveolar type II)
Loading amount
30 µg
Specification
lung alveolar type II
Treatment
shRNA treated for a period of 11 days
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Gold 30nm
Dilution
1/2000

Detection

Detection method
chemiluminescent
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 50 kDa
Positive control
No positive control used
Negative control
my samples

Additional data

Additional Notes
Whenever I use this antibody, I got two bands, one at the right size around 100, while the other at 50. I suspect maybe the 50kd band is the dimer, while the 100 band is the tetramer

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Submitted Mar 25 2009

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