Anti-Sulfatase 2/SULF2 antibody [2B4] (ab113405)
Key features and details
- Mouse monoclonal [2B4] to Sulfatase 2/SULF2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Sulfatase 2/SULF2 antibody [2B4]
See all Sulfatase 2/SULF2 primary antibodies -
Description
Mouse monoclonal [2B4] to Sulfatase 2/SULF2 -
Host species
Mouse -
Specificity
ab113405 shows no cross-reactivity with Sulfatase 1 -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Sulfatase 2/SULF2.
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Epitope
ab113405 recognises an epitope located in the C terminal domain of Sulfatase 2/SULF2. -
Positive control
- Sulfatase 2/SULF2 transfected HEK29 cells or non-small-cell Human lung carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2B4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
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Notes |
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IHC-P
1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose. |
Target
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Function
Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. -
Tissue specificity
Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 55959 Human
- SwissProt: Q8IWU5 Human
- Unigene: 162016 Human
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Alternative names
- Extracellular sulfatase Sulf 2 antibody
- Extracellular sulfatase Sulf-2 antibody
- HSulf-2 antibody
see all
Datasheets and documents
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Datasheet download
References (4)
ab113405 has been referenced in 4 publications.
- Saraswat D et al. Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination. Nat Commun 12:1923 (2021). PubMed: 33772011
- Gilhooley MJ et al. ON-bipolar cell gene expression during retinal degeneration: Implications for optogenetic visual restoration. Exp Eye Res 207:108553 (2021). PubMed: 33811915
- Zhang L et al. The upregulation of miR-204-3p in LPS-induced acute lung injury aggravated pulmonary endothelial cells apoptosis via targeting sulfatase 2. Acta Biochim Pol 68:217-222 (2021). PubMed: 34033710
- Lemjabbar-Alaoui H et al. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene 29:635-46 (2010). PubMed: 19855436