Anti-Sulfatase 2/SULF2 antibody (ab232835)
Key features and details
- Rabbit polyclonal to Sulfatase 2/SULF2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Sulfatase 2/SULF2 antibody
See all Sulfatase 2/SULF2 primary antibodies -
Description
Rabbit polyclonal to Sulfatase 2/SULF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human Sulfatase 2/SULF2 aa 300-650. N-terminal His-tag and GST-tag; Expressed in E.coli.
Database link: Q8IWU5 -
Positive control
- WB: Pig liver lysate; Recombinant human Sulfatase 2/SULF2 protein. IHC-P: Human stomach tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. -
Tissue specificity
Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 55959 Human
- SwissProt: Q8IWU5 Human
- Unigene: 162016 Human
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Alternative names
- Extracellular sulfatase Sulf 2 antibody
- Extracellular sulfatase Sulf-2 antibody
- HSulf-2 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Sulfatase 2/SULF2 using ab232835 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/ml + Pig liver lysate
Predicted band size: 100 kDa -
Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/ml + Recombinant human Sulfatase 2/SULF2 protein
Predicted band size: 100 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Sulfatase 2/SULF2 using ab232835 at 20 µg/ml in ICC/IF. FITC-labeled secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab232835 has been referenced in 1 publication.
- Li WQ et al. MicroRNA-422a functions as a tumor suppressor in non-small cell lung cancer through SULF2-mediated TGF-ß/SMAD signaling pathway. Cell Cycle 18:1727-1744 (2019). PubMed: 31204561