Key features and details
- Rabbit polyclonal to Sulfatase 2/SULF2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Sulfatase 2/SULF2 antibody
See all Sulfatase 2/SULF2 primary antibodies
DescriptionRabbit polyclonal to Sulfatase 2/SULF2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment corresponding to Human Sulfatase 2/SULF2 aa 337-649. N-terminal His-tag and GST-tag; Expressed in E.coli.
FDIRVPFYVRGPNVEAGCLNPHIVLNIDLAPTILDIAGLDIPADMDGKSI LKLLDTERPVNRFHLKKKMRVWRDSFLVERGKLLHKRDNDKVDAQEENFL PKYQRVKDLCQRAEYQTACEQLGQKWQCVEDATGKLKLHKCKGPMRLGGS RALSNLVPKYYGQGSEACTCDSGDYKLSLAGRRKKLFKKKYKASYVRSRS IRSVAIEVDGRVYHVGLGDAAQPRNLTKRHWPGAPEDQDDKDGGDFSGTG GLPDYSAANPIKVTHRCYILENDTVQCDLDLYKSLQAWKDHKLHIDHEIE TLQNKIKNLREVR
Database link: Q8IWU5
- WB: Pig liver lysate; Recombinant human Sulfatase 2/SULF2 protein. IHC-P: Human stomach tissue. ICC/IF: HepG2 cells.
This product was previously labelled as Sulfatase 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionExhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
Tissue specificityExpressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
Sequence similaritiesBelongs to the sulfatase family.
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Cellular localizationEndoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
- Information by UniProt
- Extracellular sulfatase Sulf 2 antibody
- Extracellular sulfatase Sulf-2 antibody
- HSulf-2 antibody
Formalin-fixed, paraffin-embedded human stomach tissue stained for Sulfatase 2/SULF2 using ab232835 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/ml + Pig liver lysate
Predicted band size: 100 kDa
Anti-Sulfatase 2/SULF2 antibody (ab232835) at 2 µg/ml + Recombinant human Sulfatase 2/SULF2 protein
Predicted band size: 100 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Sulfatase 2/SULF2 using ab232835 at 20 µg/ml in ICC/IF. FITC-labeled secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232835 has not yet been referenced specifically in any publications.