Sulfatase Activity Assay Kit (Colorimetric) (ab204731)

Overview

  • Product name

    Sulfatase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Product overview

    Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at OD=515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.

  • Notes

    Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    4-Nitrocatechol Standard (0.5 mM) 1 x 1.5ml
    Stop Solution Red 1 x 10ml
    Sulfatase 1 vial
    Sulfatase Assay Buffer 1 x 5ml
    Sulfatase Substrate 1 x 4ml

Images

  • Typical 4-Nitrocatechol Standard calibration curve.

  • Enzyme activity measuring different amounts of the provided Sulfatase.

  • Measurement of Sulfatase Activity in HepG2 cell lysate (30 µg) and rat liver homogenate (100 µg).

Protocols

References

ab204731 has not yet been referenced specifically in any publications.

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