Recombinant
RabMAb

Recombinant Anti-Sulfite oxidase antibody [EPR7618] (ab129094)

Overview

  • Product name

    Anti-Sulfite oxidase antibody [EPR7618]
    See all Sulfite oxidase primary antibodies
  • Description

    Rabbit monoclonal [EPR7618] to Sulfite oxidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sulfite oxidase aa 150-250.
    Database link: P51687

  • Positive control

    • Fetal kidney, HepG2, and 293T cell lysates, Human liver tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Previously labelled as SUOX.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Pathway

      Energy metabolism; sulfur metabolism.
    • Involvement in disease

      Defects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD) [MIM:272300]; also known as sulfocysteinuria. ISOD is characterized by neurological abnormalities including multicystic leukoencephalopathy with brain atrophy. Patients often suffer from seizures. Often leads to death at an early age.
    • Sequence similarities

      Contains 1 cytochrome b5 heme-binding domain.
    • Cellular localization

      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links

    • Alternative names

      • EC 1.8.3.1 antibody
      • mitochondrial antibody
      • Sulfite oxidase antibody
      • Sulfite oxidase mitochondrial antibody
      • Sulfite oxidase, mitochondrial precursor antibody
      • Suox antibody
      • SUOX_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Sulfite oxidase antibody [EPR7618] (ab129094) at 1/10000 dilution

      Lane 1 : Human fetal kidney cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa

    • ab129094, at a 1/50 dilution, staining Sulfite oxidase in paraffin embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ekman M  et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). Read more (PubMed: 24589856) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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