Sulfo-NHS-Biotin, NHS-ester biotinylation reagent (ab145609)

Overview

  • Product name

    Sulfo-NHS-Biotin, NHS-ester biotinylation reagent
  • Description

    Membrane impermeable, primary amine reactive, short chain NHS-ester biotinylation reagent
  • Biological description

    Membrane impermeable, primary amine reactive, short chain (13.5 angstroms) NHS-ester biotinylation reagent. Targets cell surface proteins. Forms irreversible amide bonds. Biotinylates antibodies.
  • Purity

    > 85%
  • CAS Number

    119616-38-5
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2,5-Dioxo-1-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoyloxy]pyrrolidine-3-sulfonate sodium salt
  • Molecular weight

    443.43
  • Molecular formula

    C14H19N3NaO8S2
  • PubChem identifier

    23700297
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Zhang WM  et al. Lactobacillus reuteri glyceraldehyde-3-phosphate dehydrogenase functions in adhesion to intestinal epithelial cells. Can J Microbiol 61:373-80 (2015). Read more (PubMed: 25867279) »
  • Cihal S & Carroll DJ Biotinylation of oocyte cell surface proteins of the starfish Patiria miniata. Methods Mol Biol 1128:197-209 (2014). Read more (PubMed: 24567216) »
  • Pintado CO  et al. The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method. Vox Sang 69:248-9 (1995). Read more (PubMed: 8578739) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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