Overview

  • Product name

    Sulfo-NHS-LC-Biotin, biotinylating reagent
  • Description

    Primary amine reactive intermediate-length biotinylating reagent
  • Biological description

    Primary amine reactive intermediate-length (22.4 angstroms) biotinylating reagent. Forms irreversible amide bonds. Negatively charged and membrane impermeable. Biotinylates cell surface proteins.
  • Purity

    > 90%
  • CAS Number

    127062-22-0
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2,5-Dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid sodium salt
  • Molecular weight

    556.59
  • Molecular formula

    C14H18O8N3S2Na
  • PubChem identifier

    16219001
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O=C2NC3C(CCCCC(=O)NCCCCCC(=O)ON1C(=O)CC(C1=O)S(=O)(=O)O[Na])SCC3N2
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Duddempudi PK  et al. Delineating the extracellular water-accessible surface of the proton-coupled folate transporter. PLoS One 8:e78301 (2013). Read more (PubMed: 24205192) »
  • Wu HN & Miyata M Whole surface image of Mycoplasma mobile, suggested by protein identification and immunofluorescence microscopy. J Bacteriol 194:5848-55 (2012). Read more (PubMed: 22923591) »
  • Yuki T  et al. Characterization of tight junctions and their disruption by UVB in human epidermis and cultured keratinocytes. J Invest Dermatol 131:744-52 (2011). Read more (PubMed: 21160495) »
See all 2 Publications for this product

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