Key features and details
- Primary amine reactive intermediate-length biotinylating reagent
- CAS Number: 127062-22-0
- Purity: > 90%
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
Product nameSulfo-NHS-LC-Biotin, biotinylating reagent
DescriptionPrimary amine reactive intermediate-length biotinylating reagent
Biological descriptionPrimary amine reactive intermediate-length (22.4 angstroms) biotinylating reagent. Forms irreversible amide bonds. Negatively charged and membrane impermeable. Biotinylates cell surface proteins.
Chemical name2,5-Dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid sodium salt
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145611 has been referenced in 2 publications.
- Lee KP et al. Functional identification of protein phosphatase 1-binding consensus residues in NBCe1-B. Korean J Physiol Pharmacol 22:91-99 (2018). PubMed: 29302216
- Suzuki Y et al. TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism. Am J Hum Genet 102:1104-1114 (2018). PubMed: 29861107