Overview

  • Product name

    Anti-Sulfotyrosine antibody [Sulfo-1C-A2]
    See all Sulfotyrosine primary antibodies
  • Description

    Mouse monoclonal [Sulfo-1C-A2] to Sulfotyrosine
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, Competitive ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human, Pig
  • Immunogen

    ab136481 was developed using a phage display library. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC

  • Positive control

    • Purified bovine fibrinogen in the absence of abalone sulfatase treatment; NIH 3T3 and HEK 293 cell lysates; Bovine cardiac tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 1.35% Glycine, 1.21% Tris, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Sulfo-1C-A2
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent dilution.
Competitive ELISA Use at an assay dependent concentration.

Images

  • All lanes : Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (ab136481) at 1/1000 dilution

    Lane 1 : Purified bovine fibrinogen treated with 1.5 mg/ml abalone sulfatase at 37°C overnight.
    Lane 2 : Purified bovine
    fibrinogen

    Secondary
    All lanes : Goat anti-mouse secondary
    antibody conjugated to HRP at 1/2000 dilution

    Developed using the ECL technique.
  • Lanes 1-3 : control IgG
    Lanes 4-6 : Anti-Sulfotyrosine antibody [Sulfo-1C-A2] (ab136481) at 1/1000 dilution

    Lanes 1 & 4 : 3T3/NIH cell lysate
    Lanes 2 & 5 : HEK 293 cell lysate
    Lanes 3 & 6 : Bovine cardiac tissue lysate

    Secondary
    All lanes : Goat anti-mouse secondary antibody conjugated to HRP at 1/2000 dilution

    Developed using the ECL technique.

References

ab136481 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136481.
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