RecombinantRabMAb

Recombinant Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

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Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
  • Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
  • Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16096] to SULT2A1/ST2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SULT2A1/ST2 antibody [EPR16096]
    See all SULT2A1/ST2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16096] to SULT2A1/ST2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human SULT2A1/ST2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06520

  • Positive control

    • HepG2, Human fetal liver, Human adrenal gland, mouse spleen, rat kidney and rat liver lysates; Human adrenal tissue; HepG2 cells.

  • General notes

     

     

     This product was previously labelled as SULT2A1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab194113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.

  • Tissue specificity

    Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.

  • Sequence similarities

    Belongs to the sulfotransferase 1 family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

Images

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human adrenal gland lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1/ST2 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

References (2)

Publishing research using ab194113? Please let us know so that we can cite the reference in this datasheet.

ab194113 has been referenced in 2 publications.

  • Wang C  et al. Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. Cell Death Dis 9:819 (2018). PubMed: 30050067
  • Wang H  et al. Glycyrrhizin and glycyrrhetinic acid inhibits alpha-naphthyl isothiocyanate-induced liver injury and bile acid cycle disruption. Toxicology 386:133-142 (2017). WB ; Mouse . PubMed: 28549656

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