Recombinant
RabMAb

Recombinant Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

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Overview

  • Product name

    Anti-SULT2A1/ST2 antibody [EPR16096]
    See all SULT2A1/ST2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16096] to SULT2A1/ST2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human SULT2A1/ST2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06520

  • Positive control

    • HepG2, Human fetal liver, Human adrenal gland, mouse spleen, rat kidney and rat liver lysates; Human adrenal tissue; HepG2 cells.

  • General notes

     

     

     This product was previously labelled as SULT2A1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.

  • Tissue specificity

    Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.

  • Sequence similarities

    Belongs to the sulfotransferase 1 family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

Images

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human adrenal gland lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1/ST2 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Wang C  et al. Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. Cell Death Dis 9:819 (2018). Read more (PubMed: 30050067) »
  • Wang H  et al. Glycyrrhizin and glycyrrhetinic acid inhibits alpha-naphthyl isothiocyanate-induced liver injury and bile acid cycle disruption. Toxicology 386:133-142 (2017). WB ; Mouse . Read more (PubMed: 28549656) »
See all 2 Publications for this product

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