Key features and details
- Rabbit polyclonal to SULT2B1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SULT2B1 antibody
See all SULT2B1 primary antibodies
DescriptionRabbit polyclonal to SULT2B1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human placenta tissue. ICC/IF: U-2 OS cells. WB: Human tonsil lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab254616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
Tissue specificityExpressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
Sequence similaritiesBelongs to the sulfotransferase 1 family.
- Information by UniProt
- Alcohol sulfotransferase antibody
- EC 220.127.116.11 antibody
- HSST2 antibody
Paraffin-embedded human placenta tissue stained for SULT2B1 using ab254616 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-SULT2B1 antibody (ab254616) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 (human urinary bladder cancer cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 41 kDa
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling SULT2B1 using ab254616 at 4µg/ml (green) in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254616 has not yet been referenced specifically in any publications.