Product nameAnti-SULT2B1 antibody
See all SULT2B1 primary antibodies
DescriptionRabbit polyclonal to SULT2B1
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
- Transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab82865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay is 1/62500.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCatalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
Tissue specificityExpressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
Sequence similaritiesBelongs to the sulfotransferase 1 family.
- Information by UniProt
- Alcohol sulfotransferase antibody
- EC 188.8.131.52 antibody
- HSST2 antibody
Anti-SULT2B1 antibody (ab82865) at 1 µg/ml (5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Gel concentration: 12%
Anti-SULT2B1 antibody (ab82865) at 1/1000 dilution + whole cell lysate prepared from murine macrophages at 30 µg
HRP conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Segala G et al. 5,6-Epoxy-cholesterols contribute to the anticancer pharmacology of Tamoxifen in breast cancer cells. Biochem Pharmacol 86:175-89 (2013). Read more (PubMed: 23500540) »
- Yang X et al. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo. PLoS One 8:e60853 (2013). WB, ICC/IF ; Mouse, Human . Read more (PubMed: 23593328) »