Anti-FGE antibody (ab91479)
Key features and details
- Goat polyclonal to FGE
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-FGE antibody -
Description
Goat polyclonal to FGE -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human FGE aa 314-327 (internal sequence).
Sequence:C-ETLNPKGPPSGKDR
Database link: NP_877437.2 -
Positive control
- Mouse pancreas, Mouse eye and Human kidney lysates
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab91479 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. -
Tissue specificity
Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart. -
Pathway
Protein modification; sulfatase oxidation. -
Involvement in disease
Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the sulfatase-modifying factor family. -
Post-translational
modificationsN-glycosylated. Contains high-mannose-type oligosaccharides. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 536435 Cow
- Entrez Gene: 484681 Dog
- Entrez Gene: 58911 Mouse
- Entrez Gene: 362409 Rat
- SwissProt: Q0P5L5 Cow
- SwissProt: Q8R0F3 Mouse
- Unigene: 439876 Mouse
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Alternative names
- MGC150436 antibody
- AAPA3037 antibody
- C alpha formylglycine generating enzyme 1 antibody
see all
Images
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Anti-FGE antibody (ab91479) at 1 µg/ml + Mouse Pancreas lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab91479 has not yet been referenced specifically in any publications.