Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
Key features and details
- Biotin Rat monoclonal [4D12] to Sumo 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
-
Product name
Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin)
See all Sumo 1 primary antibodies -
Description
Biotin Rat monoclonal [4D12] to Sumo 1 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein (proprietary-tag) corresponding to Human Sumo 1.
Database link: P63165 -
Positive control
- HeLa total cell extract; mouse primary culture neurons; C-33A Human cervix carcinoma cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Store In the Dark. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
-
Purity
Proprietary Purification -
Purification notes
ab179907 was produced in serum-free medium and purified under mild conditions by propriety chromatography processes and conjugated with biotin. ab179907 is filter-sterilised. -
Clonality
Monoclonal -
Clone number
4D12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. -
Involvement in disease
Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays. -
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsCleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4. -
Cellular localization
Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body. - Information by UniProt
-
Database links
- Entrez Gene: 7341 Human
- Entrez Gene: 22218 Mouse
- Omim: 601912 Human
- SwissProt: P63165 Human
- SwissProt: P63166 Mouse
- Unigene: 596171 Human
- Unigene: 81424 Human
- Unigene: 362118 Mouse
-
Alternative names
- DAP1 antibody
- GAP modifying protein 1 antibody
- GAP-modifying protein 1 antibody
see all
Images
-
Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179907 at 10 ug/ml
-
Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907) + HeLa total cell extract
Predicted band size: 11 kDaAn 80 kDa single and other multiple bands were observed in HeLa total cell extract at 1 ug/ml
-
Immunofluorescent analysis of mouse primary culture neurons labeling Sumo 1 with ab179907 (left panel) at 10 ug/ml. DNA was stained with Hoechst (right panel).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab179907 has been referenced in 2 publications.
- Uchimura Y et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem 281:23180-90 (2006). PubMed: 16757475
- Saitoh N et al. In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. Exp Cell Res 312:1418-30 (2006). PubMed: 16688858