Overview

  • Product name

    Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin)
    See all Sumo 1 primary antibodies
  • Description

    Rat monoclonal [4D12] to Sumo 1 - BSA and Azide free (Biotin)
  • Host species

    Rat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant full length protein (proprietary-tag) corresponding to Human Sumo 1.
    Database link: P63165

  • Positive control

    • HeLa total cell extract; mouse primary culture neurons; C-33A Human cervix carcinoma cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab179907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
  • Involvement in disease

    Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
  • Sequence similarities

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications

    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
  • Cellular localization

    Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAP1 antibody
    • GAP modifying protein 1 antibody
    • GAP-modifying protein 1 antibody
    • GMP 1 antibody
    • GMP1 antibody
    • OFC10 antibody
    • PIC 1 antibody
    • PIC1 antibody
    • SENP2 antibody
    • Sentrin 1 antibody
    • Sentrin antibody
    • Small ubiquitin related modifier 1 antibody
    • Small ubiquitin-like modifier 1 antibody
    • Small ubiquitin-related modifier 1 antibody
    • SMT3 antibody
    • SMT3 homolog 3 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3, yeast, homolog 3 antibody
    • Smt3C antibody
    • SMT3H3 antibody
    • SUMO-1 antibody
    • SUMO1 antibody
    • SUMO1_HUMAN antibody
    • Ubiquitin homology domain protein PIC1 antibody
    • Ubiquitin Like 1 antibody
    • Ubiquitin like protein SMT3C antibody
    • Ubiquitin like protein UBL1 antibody
    • Ubiquitin-homology domain protein PIC1 antibody
    • Ubiquitin-like protein SMT3C antibody
    • Ubiquitin-like protein UBL1 antibody
    • UBL 1 antibody
    • UBL1 antibody
    see all

Images

  • Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179907 at 10 ug/ml

  • Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907) + HeLa total cell extract

    Predicted band size: 11 kDa



    An 80 kDa single and other multiple bands were observed in HeLa total cell extract at 1 ug/ml

  • Immunofluorescent analysis of mouse primary culture neurons labeling Sumo 1 with ab179907 (left panel) at 10 ug/ml. DNA was stained with Hoechst (right panel).

References

This product has been referenced in:

  • Uchimura Y  et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem 281:23180-90 (2006). Read more (PubMed: 16757475) »
  • Saitoh N  et al. In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. Exp Cell Res 312:1418-30 (2006). Read more (PubMed: 16688858) »
See all 2 Publications for this product

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