• Product name

  • Description

    Rabbit polyclonal to Sumo 1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human, Escherichia coli
  • Immunogen

    Recombinant his-tagged full length soluble Sumo 1 (Human).



Our Abpromise guarantee covers the use of ab11672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21317883
WB 1/1000 - 1/5000. Predicted molecular weight: 12 kDa. For 1 hour 30 minutes at RT in 5% milk in TBST (note: 0.7% Tween).
A 1/5000 dilution of the antibody will detect 0.1ng of the recombinant protein used as an immunogen.
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.


  • Function

    Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
  • Involvement in disease

    Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
  • Sequence similarities

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational

    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
  • Cellular localization

    Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAP1 antibody
    • GAP modifying protein 1 antibody
    • GAP-modifying protein 1 antibody
    • GMP 1 antibody
    • GMP1 antibody
    • OFC10 antibody
    • PIC 1 antibody
    • PIC1 antibody
    • SENP2 antibody
    • Sentrin 1 antibody
    • Sentrin antibody
    • Small ubiquitin related modifier 1 antibody
    • Small ubiquitin-like modifier 1 antibody
    • Small ubiquitin-related modifier 1 antibody
    • SMT3 antibody
    • SMT3 homolog 3 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3, yeast, homolog 3 antibody
    • Smt3C antibody
    • SMT3H3 antibody
    • SUMO-1 antibody
    • SUMO1 antibody
    • SUMO1_HUMAN antibody
    • Ubiquitin homology domain protein PIC1 antibody
    • Ubiquitin Like 1 antibody
    • Ubiquitin like protein SMT3C antibody
    • Ubiquitin like protein UBL1 antibody
    • Ubiquitin-homology domain protein PIC1 antibody
    • Ubiquitin-like protein SMT3C antibody
    • Ubiquitin-like protein UBL1 antibody
    • UBL 1 antibody
    • UBL1 antibody
    see all


  • Immunofluorescence of 293T cells transfected with a vector that has Sumo1 fused to GFP and a Flag tag. The top panel is GFP fluorescence. The middle panel uses ab11672 at 1/300 with a rhodamine secondary antibody. The lower panel is a merge of the GFP fluorescence and ab11672 immunostaining. Staining was also seen in untransfected cells (although at a lower level).

  • 293T cells were transfected with a vector that has Sumo1 fused to GFP and a Flag tag. Cell lysates were used in IP with ab11672 (and a GFP antibody as a control). The resulting western blot was performed with a Flag antibody. As a control, cells were transfected with a a vector with Sumo2 fused to GFP and a Flag tag. ab11672 does not IP anything from this lysate.

    Lane 1: Sumo1 fusion lysate - IP'd with GFP antibody

    Lane 2: Sumo1 fusion lysate - no IP

    Lane 3: Sumo1 fusion lysate - IP'd with ab11672

    Lane 4: Sumo2 fusion lysate - IP'd with ab11672

  • Ab11672 staining human normal placenta. Staining is localized to the nucleus and nuclear membrane.
    Left panel: with primary whole serum antibody at 1/400. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be
  • ICC/IF image of ab11672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11672, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence analysis of WM-266-4 cells. Left panel = untreated cells. Right panel = cells treated for 3 h with 25 ng/ml leptomycin B and for 45 min with 50 ng/ml NRG-1. Sumo 1 was stained with ab11672 (green).


This product has been referenced in:

  • Guion L  et al. PML nuclear body-residing proteins sequentially associate with HPV genome after infectious nuclear delivery. PLoS Pathog 15:e1007590 (2019). IF ; Human . Read more (PubMed: 30802273) »
  • Hernandez I  et al. A farnesyltransferase inhibitor activates lysosomes and reduces tau pathology in mice with tauopathy. Sci Transl Med 11:N/A (2019). ICC ; Mouse . Read more (PubMed: 30918111) »
See all 22 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

IHC - Wholemount
Mouse Tissue (Brain)

Mr. Gabriel Luna

Verified customer

Submitted Jan 08 2018


I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Human Cell lysate - whole cell (HCT116)
Total protein in input
500 µg
transfected with sumoylated protein
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Jun 06 2011

Western blot
Escherichia coli Recombinant protein (GST purified protein)
Loading amount
0.01 µg
GST purified protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C

Dr. Sriharsha Kantamneni

Verified customer

Submitted Aug 28 2007

Western blot
Human Recombinant protein (Sumoylation assay of P.O.I.)
Loading amount
0.0125 µg
Sumoylation assay of P.O.I.
Sumoylation Assay 3hrs.
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Don Anderson

Verified customer

Submitted Aug 23 2006


We forwarded your protocol to the source of the antibody and we have just received this information: " I do agree that the anti-Sumo antibody it present a basal background in the western blot, but at least in the conditions used in the lab, we always have been able to see the Sumo band. However, due to the enquiry, I performed new conditions to try to improve the signal to background ratio. The conditions used for this western blot are similar to the conditions used by the user but changing several things: 1- dilution of the primary: 1:3000 2- in all the incubations TBST-0.7 (Tween: 0.7% !!!, yes, 0.7%) was used. Other details that might be important: 3- incubation time for the antibody: 1h 30 min at RT 4- membrane used for the transference: nitrocellulose (Scheleicher & Schuell, Protran BA 85, pore size: 0.45 um) 5- might be important the amount of extract added: in the figure it was loaded around 50 ug of total cell extracts 6- we used west pico luminol products by pierce... very sensitive ECL might give a strong background. (the rest it is pretty much the same as described by the user: primary and secondary diluted in TBST-0.7 + 5% skimmed milk)." I hope this helps you, if you still have problems please get in touch with us,

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I'm very sorry for the delay in replying to you, we have been trying to get in touch with the academic who has provided us with ab11672 and unfortunately it has proven very difficult. We are still trying to find out a recommended protocol, my sincere apologies for the delay. I would like to ask if you have tried a different blocking agent, like BSA 5% or serum? If after testing those you still have the same problem I will of course send you a replacement antibody. I look forward to hearing from you, thank you for your patience,

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