Name: Anti-Sumo 1 antibody [EP298]
Brand: RabMAb
SKU: ab133352
Rating: 5 (2 reviews)

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Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP298] to Sumo 1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Sumo 1 antibody [EP298]
See all Sumo 1 primary antibodies
Description

Rabbit monoclonal [EP298] to Sumo 1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Sumo 1 aa 1-100. The exact sequence is proprietary.
Positive control
- WB- HeLa whole cell lysate (ab150035), MCF-7 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, C6 whole cell lysate, HL-60 cell lysate and A431 cell lysate. IHC- Human papillary cell thyroid gland carcinoma tissue, Human Breast carcinoma tissue, Human Glioma tissue, Human Urinary bladder transitional carcinoma tissue and Human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP298
Isotype

IgG
Research areas

Alternative Versions
- Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
Compatible Secondaries
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(1)	1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Predicted molecular weight: 11.5 kDa.

Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 11.5 kDa.

Application notes

Is unsuitable for Flow Cyt.

Function

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
Involvement in disease

Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
Sequence similarities

Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
Post-translational
modifications

Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
Cellular localization

Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
Target information above from: UniProt accession P63165 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7341 Human
- Entrez Gene: 22218 Mouse
- Entrez Gene: 301442 Rat
- Omim: 601912 Human
- SwissProt: P63165 Human
- SwissProt: P63166 Mouse
- SwissProt: Q5I0H3 Rat
- Unigene: 596171 Human
- Unigene: 81424 Human
- Unigene: 362118 Mouse
- Unigene: 1221 Rat
see all
Alternative names
- DAP1 antibody
- GAP modifying protein 1 antibody
- GAP-modifying protein 1 antibody
- GMP 1 antibody
- GMP1 antibody
- OFC10 antibody
- PIC 1 antibody
- PIC1 antibody
- SENP2 antibody
- Sentrin 1 antibody
- Sentrin antibody
- Small ubiquitin related modifier 1 antibody
- Small ubiquitin-like modifier 1 antibody
- Small ubiquitin-related modifier 1 antibody
- SMT3 antibody
- SMT3 homolog 3 antibody
- SMT3 suppressor of mif two 3 homolog 1 antibody
- SMT3, yeast, homolog 3 antibody
- Smt3C antibody
- SMT3H3 antibody
- SUMO-1 antibody
- SUMO1 antibody
- SUMO1_HUMAN antibody
- Ubiquitin homology domain protein PIC1 antibody
- Ubiquitin Like 1 antibody
- Ubiquitin like protein SMT3C antibody
- Ubiquitin like protein UBL1 antibody
- Ubiquitin-homology domain protein PIC1 antibody
- Ubiquitin-like protein SMT3C antibody
- Ubiquitin-like protein UBL1 antibody
- UBL 1 antibody
- UBL1 antibody
see all

Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Sumo 1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF-7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133352 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133352 was shown to recognize Sumo 1 when Sumo 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. ab133352 and ab8245 (loading control to GAPDH) were diluted 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical staining of paraffin-embedded human thyroid carcinoma sections labelling Sumo 1 with purified ab133352 at dilution of 1:100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/10000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 5 : C6 (rat glioma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 11.5 kDa
Observed band size: 18-90 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11.5 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin-embedded Human papillary cell thyroid gland carcinoma tissue labelling Sumo 1 with un-purified ab133352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 11.5 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Sumo 1 antibody [EP298] (ab133352)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab133352? Please let us know so that we can cite the reference in this datasheet.

ab133352 has been referenced in 7 publications.

Liu Y et al. Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle. Exp Ther Med 19:1855-1863 (2020). PubMed: 32104241
Carmichael RE et al. Insulin-dependent GLUT4 trafficking is not regulated by protein SUMOylation in L6 myocytes. Sci Rep 9:6477 (2019). PubMed: 31019221
Tan F et al. Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia. Mol Med Rep 17:1283-1288 (2018). PubMed: 29115559
Liu F et al. Overexpression of SENP1 reduces the stemness capacity of osteosarcoma stem cells and increases their sensitivity to HSVtk/GCV. Int J Oncol 53:2010-2020 (2018). PubMed: 30226577
Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
Liu X et al. Hypothermia inhibits the proliferation of bone marrow-derived mesenchymal stem cells and increases tolerance to hypoxia by enhancing SUMOylation. Int J Mol Med 40:1631-1638 (2017). PubMed: 29039464
Jester JV et al. PPAR? Regulates Mouse Meibocyte Differentiation and Lipid Synthesis. Ocul Surf 14:484-494 (2016). PubMed: 27531629

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HAP1)

Permeabilization

Yes - 0.5% Triton 5 minutes

Specification

HAP1

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10%

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (7)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 1 antibody [EP298]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sumo 1 antibody [EP298]

Post-translational
modifications