Validated using a knockout cell line

Recombinant Anti-Sumo 1 antibody [Y299] (ab32058)

Knockout Tested Rabbit recombinant monoclonal Sumo 1 antibody [Y299]. Validated in WB, IP, IHC, Flow Cyt, ChIP, ICC/IF and tested in Mouse, Rat, Human. Cited in 48 publication(s).

Publishing research using ab32058? Please let us know so that we can cite the reference in this datasheet.

ab32058 has been referenced in 53 publications.

  • Gómez-Molina C  et al. Small Extracellular Vesicles in Rat Serum Contain Astrocyte-Derived Protein Biomarkers of Repetitive Stress. Int J Neuropsychopharmacol 22:232-246 (2019). PubMed: 30535257
  • Wang QL  et al. T Cell Receptor (TCR)-Induced PLC-?1 Sumoylation via PIASxß and PIAS3 SUMO E3 Ligases Regulates the Microcluster Assembly and Physiological Function of PLC-?1. Front Immunol 10:314 (2019). PubMed: 30873169
  • Heras G  et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). PubMed: 29868881
  • Chen Q  et al. Sumoylation of EphB1 Suppresses Neuroblastoma Tumorigenesis via Inhibiting PKC? Activation. Cell Physiol Biochem 45:2283-2292 (2018). PubMed: 29550816
  • Sampaio EP  et al. Novel signal transducer and activator of transcription 1 mutation disrupts small ubiquitin-related modifier conjugation causing gain of function. J Allergy Clin Immunol 141:1844-1853.e2 (2018). PubMed: 28859974
  • Yuan H  et al. SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis. Mol Cancer 16:157 (2017). PubMed: 29020972
  • Zitti B  et al. Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells. Sci Rep 7:10445 (2017). WB ; Human . PubMed: 28874810
  • Wang J  et al. Src SUMOylation Inhibits Tumor Growth Via Decreasing FAK Y925 Phosphorylation. Neoplasia 19:961-971 (2017). PubMed: 29069627
  • Huang W  et al. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-?B Inflammatory Signaling in Rat Mesangial Cells. Mediators Inflamm 2017:1685194 (2017). PubMed: 29038616
  • Yang WS  et al. SUMO modification of a heterochromatin histone demethylase JMJD2A enables viral gene transactivation and viral replication. PLoS Pathog 13:e1006216 (2017). WB ; Human . PubMed: 28212444
  • Cloutier JM  et al. Mammalian meiotic silencing exhibits sexually dimorphic features. Chromosoma 125:215-26 (2016). PubMed: 26712235
  • Conn KL  et al. Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. J Virol 90:4807-26 (2016). PubMed: 26937035
  • Ghosh H  et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun 7:13365 (2016). PubMed: 27819299
  • Maroui MA  et al. Latency Entry of Herpes Simplex Virus 1 Is Determined by the Interaction of Its Genome with the Nuclear Environment. PLoS Pathog 12:e1005834 (2016). PubMed: 27618691
  • Wang F  et al. Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations. Osteoarthritis Cartilage 24:1113-24 (2016). PubMed: 26826302
  • Xiao Y  et al. Inhibition of CDK1 activity by sumoylation. Biochem Biophys Res Commun 478:919-23 (2016). PubMed: 27520372
  • Xiao Y  et al. Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins. Anal Biochem 477:95-7 (2015). PubMed: 25454506
  • Yeo AJ  et al. Senataxin controls meiotic silencing through ATR activation and chromatin remodeling. Cell Discov 1:15025 (2015). WB, IF . PubMed: 27462424
  • Bish R  et al. Comprehensive Protein Interactome Analysis of a Key RNA Helicase: Detection of Novel Stress Granule Proteins. Biomolecules 5:1441-66 (2015). PubMed: 26184334
  • Chen C  et al. SUMOylation of TARBP2 regulates miRNA/siRNA efficiency. Nat Commun 6:8899 (2015). PubMed: 26582366
  • Yang WS  et al. K-bZIP Mediated SUMO-2/3 Specific Modification on the KSHV Genome Negatively Regulates Lytic Gene Expression and Viral Reactivation. PLoS Pathog 11:e1005051 (2015). ChIP . PubMed: 26197391
  • Deng R  et al. Shp2 SUMOylation promotes ERK activation and hepatocellular carcinoma development. Oncotarget 6:9355-69 (2015). PubMed: 25823821
  • Li W  et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). PubMed: 25644173
  • Sidik SM  et al. Shigella infection interferes with SUMOylation and increases PML-NB number. PLoS One 10:e0122585 (2015). WB . PubMed: 25848798
  • Everett RD  et al. Sequences related to SUMO interaction motifs in herpes simplex virus 1 protein ICP0 act cooperatively to stimulate virus infection. J Virol 88:2763-74 (2014). PubMed: 24352468
  • Geerts CJ  et al. Tomosyn interacts with the SUMO E3 ligase PIAS?. PLoS One 9:e91697 (2014). WB ; Human . PubMed: 24614299
  • Radko S  et al. Adenovirus E1A targets the DREF nuclear factor to regulate virus gene expression, DNA replication, and growth. J Virol 88:13469-81 (2014). IF ; Mouse . PubMed: 25210186
  • Katyal S  et al. Aberrant topoisomerase-1 DNA lesions are pathogenic in neurodegenerative genome instability syndromes. Nat Neurosci 17:813-21 (2014). PubMed: 24793032
  • Kalkat M  et al. Identification of c-MYC SUMOylation by mass spectrometry. PLoS One 9:e115337 (2014). WB ; Human . PubMed: 25522242
  • Poulsen SL  et al. RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response. J Cell Biol 201:797-807 (2013). PubMed: 23751493
  • Vigodner M  et al. Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa. Hum Reprod 28:210-23 (2013). Human . PubMed: 23077236
  • Balasubramaniyan N  et al. SUMOylation of the farnesoid X receptor (FXR) regulates the expression of FXR target genes. J Biol Chem 288:13850-62 (2013). PubMed: 23546875
  • Chang PC  et al. The chromatin modification by SUMO-2/3 but not SUMO-1 prevents the epigenetic activation of key immune-related genes during Kaposi's sarcoma associated herpesvirus reactivation. BMC Genomics 14:824 (2013). ChIP . PubMed: 24267727
  • Glass M & Everett RD Components of promyelocytic leukemia nuclear bodies (ND10) act cooperatively to repress herpesvirus infection. J Virol 87:2174-85 (2013). PubMed: 23221561
  • Santos A  et al. SUMOylation affects the interferon blocking activity of the influenza A nonstructural protein NS1 without affecting its stability or cellular localization. J Virol 87:5602-20 (2013). PubMed: 23468495
  • Cai Q  et al. A Unique SUMO-2-Interacting Motif within LANA Is Essential for KSHV Latency. PLoS Pathog 9:e1003750 (2013). Human . PubMed: 24278015
  • Gómez-Pastor R  et al. Trx2p-dependent regulation of Saccharomyces cerevisiae oxidative stress response by the Skn7p transcription factor under respiring conditions. PLoS One 8:e85404 (2013). WB . PubMed: 24376879
  • Ismail IH  et al. CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res 40:5497-510 (2012). ICC/IF ; Human . PubMed: 22402492
  • Neumeister VM  et al. Quantitative assessment of effect of preanalytic cold ischemic time on protein expression in breast cancer tissues. J Natl Cancer Inst 104:1815-24 (2012). PubMed: 23090068
  • Stanton PG  et al. Proteomic changes in rat spermatogenesis in response to in vivo androgen manipulation; impact on meiotic cells. PLoS One 7:e41718 (2012). IHC . PubMed: 22860010
  • Zhang LJ  et al. Coordinated regulation of transcription factor Bcl11b activity in thymocytes by the mitogen-activated protein kinase (MAPK) pathways and protein sumoylation. J Biol Chem 287:26971-88 (2012). PubMed: 22700985
  • Plant LD  et al. SUMO modification of cell surface Kv2.1 potassium channels regulates the activity of rat hippocampal neurons. J Gen Physiol 137:441-54 (2011). ICC . PubMed: 21518833
  • De Vos WH  et al. Repetitive disruptions of the nuclear envelope invoke temporary loss of cellular compartmentalization in laminopathies. Hum Mol Genet 20:4175-86 (2011). PubMed: 21831885
  • Gibbings S  et al. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-{gamma} sumoylation, and HIF-1{alpha}. J Biol Chem 286:961-75 (2011). WB ; Human . PubMed: 21059659
  • Cuchet-Lourenço D  et al. SUMO Pathway Dependent Recruitment of Cellular Repressors to Herpes Simplex Virus Type 1 Genomes. PLoS Pathog 7:e1002123 (2011). ICC/IF ; Human . PubMed: 21779164
  • Boutell C  et al. A Viral Ubiquitin Ligase Has Substrate Preferential SUMO Targeted Ubiquitin Ligase Activity that Counteracts Intrinsic Antiviral Defence. PLoS Pathog 7:e1002245 (2011). WB ; Human . PubMed: 21949651
  • Pal S  et al. Influenza A virus interacts extensively with the cellular SUMOylation system during infection. Virus Res 158:12-27 (2011). WB ; Human . PubMed: 21376763
  • Cuchet D  et al. PML isoforms I and II participate in PML-dependent restriction of HSV-1 replication. J Cell Sci 124:280-91 (2011). WB ; Human . PubMed: 21172801
  • Driscoll JJ  et al. The sumoylation pathway is dysregulated in multiple myeloma and is associated with adverse patient outcome. Blood 115:2827-34 (2010). WB ; Human . PubMed: 19965618
  • Plant LD  et al. One SUMO is sufficient to silence the dimeric potassium channel K2P1. Proc Natl Acad Sci U S A 107:10743-8 (2010). ICC/IF ; Chinese hamster . PubMed: 20498050
  • Cai Q  et al. Hypoxia inactivates the VHL tumor suppressor through PIASy-mediated SUMO modification. PLoS One 5:e9720 (2010). PubMed: 20300531
  • Vigodner M  et al. Differential expression patterns of SUMO proteins in HL-60 cancer cell lines support a role for sumoylation in the development of drug resistance. Cell Tissue Res 336:277-86 (2009). ICC/IF ; Human . PubMed: 19326147
  • van Niekerk EA  et al. Sumoylation in axons triggers retrograde transport of the RNA-binding protein La. Proc Natl Acad Sci U S A 104:12913-8 (2007). PubMed: 17646655

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