Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 12-250 kDa
Additional data
Additional Notes
blot shows formation of high molecular weight bands consistent with the formation of SUMO1 conjugated / SUMO1ylated proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jul 01 2016