Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Permeabilization
Yes - 0.1% Triton-X
Specification
Spinal cord
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
Thank you for taking part in our Abtrial program and your valuable feedback. We are pleased to see that the antibody is working well for you in IHC-Fr, an application we have not tested before. We welcome further feedback and AbReviews from other customers who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jul 21 2016