Application
Western blot
Sample
Human Cell lysate - whole cell (head and neck cancer cell)
Gel Running Conditions
Reduced Denaturing (8% SDS page gel)
Loading amount
30 µg
Treatment
5uM DIM-C-pPhtBu for 24h
Specification
head and neck cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim milk ( in PBST )
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL™ Select Western Blotting Detection R
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 24 2018