Recombinant Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)
![Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.com/ps/products/109/ab109196/Images/ab109196-121816-anti-sumo-23-antibody-epr3002-western-blot.jpg "Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)]
See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies -
Description
Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Sumo 2 + Sumo 3 aa 1-100. The exact sequence is proprietary. The immunogen sequence is based upon a region found in both Sumo 2 and Sumo 3 that has significant homology with Sumo 4.
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Positive control
- Paraffin-embedded Human ovarian tissue HeLa and Jurkat cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR300(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 12 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Images
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Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.com/ps/products/109/ab109196/Images/ab109196-121815-anti-sumo-23-antibody-epr3002-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)ab109196, at 1/100, staining Sumo 2 + Sumo 3 + Sumo 4 in paraffin-embedded Human ovarian tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.com/ps/products/109/ab109196/Images/ab109196-451126-anti-sumo-2-sumo-3-sumo-4-antibody-epr300-2-western-blot-recombinant-human.jpg)
Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/200 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 :Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
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![Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.com/ps/products/109/ab109196/Images/ab109196-3-benefits-of-recombinant-antibodies.png)
Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)
Protocols
References (0)
ab109196 has not yet been referenced specifically in any publications.
