Recombinant Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free
See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies -
Description
Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247789 is the carrier-free version of ab109196 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247789 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR300(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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Notes |
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ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
Images
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All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDaThis data was developed using ab109196, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
This data was developed using ab109196, the same antibody clone in a different buffer formulation.
ab109196, at 1/100, staining Sumo 2 + Sumo 3 + Sumo 4 in paraffin-embedded Human ovarian tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/200 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 :Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
This data was developed using ab109196, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab247789 has not yet been referenced specifically in any publications.