Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163]
See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies
DescriptionRabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Sumo 2 + Sumo 3 + Sumo 4 aa 50-150 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region in Sumo 4 that has significant homology with Sumo 2 and Sumo 3.
Database link: Q6EEV6
- HepG3, 293T and Jurkat whole cell lysate (ab7899); Human spleen tissue lysate; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.82 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa).|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab126606 at 1/250 dilution staining Sumo 2 + Sumo 3 + Sumo 4 in HepG2 (Human liver hepatocellular carcinoma cell line) cells by immunofluorescence.
All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa
All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/5000 dilution
Lane 1 :
Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :
Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 :
Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :
Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Blocking buffer: 5% NFDM/TBST
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126606 has been referenced in 3 publications.
- Liang JX et al. SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells. Oncol Lett 20:225 (2020). PubMed: 32968447
- Ma X et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970
- Tan F et al. Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia. Mol Med Rep 17:1283-1288 (2018). PubMed: 29115559