Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371)

Mouse monoclonal Sumo 2+3 antibody [8A2]. Validated in WB, IP, IHC, ChIP, ICC/IF and tested in Human. Cited in 28 publication(s). Independently reviewed in 6 review(s).

Overview

  • Product name

    Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade
    See all Sumo 2 + Sumo 3 primary antibodies
  • Description

    Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 - ChIP Grade
  • Host species

    Mouse
  • Specificity

    This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.
  • Tested applications

    Suitable for: WB, ICC/IF, IP, ChIP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Sumo 2 + Sumo 3.

  • Positive control

    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab81371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 12 kDa.
ICC/IF 1/2000.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • Cellular localization

    Cytoplasmic (SUMO3) and Nuclear (SUMO2)
  • Database links

  • Alternative names

    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin like modifier 3 antibody
    • Small ubiquitin related modifier 2 antibody
    • Small ubiquitin related modifier 3 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    • Ubiquitin-like protein SMT3B antibody
    see all

Images

  • Anti-Sumo 2 + Sumo 3 antibody [8A2] - ChIP Grade (ab81371) at 1/2000 dilution + HeLa cell lysate

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Ab81371 (1:2000) staining human Sumo 2 + 3 in HeLa cells by immunofluorescence.
  • IHC image of ab81371 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81371, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Borgermann N  et al. SUMOylation promotes protective responses to DNA-protein crosslinks. EMBO J 38:N/A (2019). Read more (PubMed: 30914427) »
  • Liebelt F  et al. SUMOylation and the HSF1-Regulated Chaperone Network Converge to Promote Proteostasis in Response to Heat Shock. Cell Rep 26:236-249.e4 (2019). Read more (PubMed: 30605679) »
See all 30 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 0.1% · Temperature: 25°C
Sample
Rat Cell (Glial cells)
Specification
Glial cells
Permeabilization
Yes - 0.01% Saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 10 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (Mixed glial cells)
Specification
Mixed glial cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 30 2014

Answer

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 12 months of purchase.

So my suggestion would be to try the antibody and if not completely satisfied we will either replace the antibody or give you a different product to try.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus egg extract)
Loading amount
40 µg
Specification
Xenopus egg extract
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Andrew Lane

Verified customer

Submitted Apr 11 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Nuclei generated in oocyte extract)
Specification
Nuclei generated in oocyte extract
Fixative
Formaldehyde
Permeabilization
Yes - PBS-0.1% NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Dr. Andrew Lane

Verified customer

Submitted Apr 01 2013

Question
Answer

Thank you for contacting us.
Both ab3742 and ab81371 have immunogen with 100% homology with the mouse sequence.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry and your interest in our products.
This particular antibody is not affinity purified but ascitic fluid so the concentration is not determined. As a general guidance, the concentration range is between: 5 - 10 mg/ml and the recommended dilution factor for IP would be 1/100. You may wish to take a look at this Table for further details:
https://www.abcam.com/index.html?pageconfig=technicalfaqs#8
If an antibody is ChIP grade, it means that it has been specifically tested in ChIP application in the Lab or we have specific published data available for the product so is suitable for this application.
If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Application
ChIP
Sample
Human Cell lysate - whole cell (liver)
Specification
liver
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Detection step
Semiquantitative PCR
Positive control
pol 11
Negative control
IgG

Mr. Natarajan Balasubramaniyan

Verified customer

Submitted May 19 2012

Question

Dear Dr Keith Yes,I want use in the beginning ab32219 and ab32058 for the ChiP assay in human liver cell line.Based on the results I want use other antibody as well.I am going to order all the antibody now.Thanks. With regards Bala ________________________________________ From: technical@abcam.com [technical@abcam.com] Sent: Thursday, January 12, 2012 3:27 PM To: Balasubramaniyan, Natarajavelalar Subject: Reply from Abcam to your enquiry regarding ab32058, ab32219, ab75854, ab81371 [CCE3421816] [https://www.abcam.com/ps/cms/emails/CRM-Header.jpg] Dear Sir/Madam We have an answer to your inquiry: Thank you very much for your interest in ab32219, ab32058, ab81371, ab75854. To our knowledge, these products has not been tested in ChIP on human. Therefore, I can offer a discount off a future purchase if you buy now, test in ChIP on human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test any or each of these products in ChIP on human. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchase the antibody or antibodies either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Help us improve our service. Rate your experience with us today. Your original inquiry to Abcam: Will these work on ChIP as well? Best regards, Keith Keith Beadle Scientific Support Specialist Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team www.abcam.com/technical [CCE3421816] Discover more at abcam.com

Read More
Answer

Ab32058 DISCOUNT CODE: Expiration date: Ab32058 DISCOUNT CODE: Expiration date: Ab81371 DISCOUNT CODE: Expiration date: 14 May 2012 Ab75854 DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and testthese productsinChIP. This code will give you 1 free PRIMARY ANTIBODYper codebefore the expiration date. To redeem this offer, please submit an Abreview foreach product in ChIPand include the associatedcode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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1-10 of 11 Abreviews or Q&A

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