Key features and details
- Rabbit polyclonal to Sumo 2 + Sumo 3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Sumo 2 + Sumo 3 antibody
See all Sumo 2 + Sumo 3 primary antibodies
DescriptionRabbit polyclonal to Sumo 2 + Sumo 3
This antibody recognizes Human Sumo 2 and Sumo 3.
This antibody shows no cross-reactivity with related proteins SUMO1, UCRP, Nedd8, and FAT10 in Western blotting.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Xenopus laevis, Zebrafish
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Purification notesThis antibody has been partially purified by salt precipitation.
Our Abpromise guarantee covers the use of ab22654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa). Dilute to working strength with 50mM sodium phosphate buffer (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).|
RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
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SUMOylation assay utilising His6-tagged SUMO 2, SUMO E1, SUMO E2 and RANGAP1 as substrate in presence (lane 2) and absence (lanes (1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with ab22654. SUMOylation assay utilising His6-tagged SUMO 2, SUMO E1, SUMO E2 and RANGAP1 as substrate in presence (lane 2) and absence (lanes (1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with ab22654.
IHC image of ab22654 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22654, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab22654 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22654, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab22654 has been referenced in 4 publications.
- Alandijany T et al. Distinct temporal roles for the promyelocytic leukaemia (PML) protein in the sequential regulation of intracellular host immunity to HSV-1 infection. PLoS Pathog 14:e1006769 (2018). PubMed: 29309427
- Conn KL et al. Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. J Virol 90:4807-26 (2016). PubMed: 26937035
- Sabò A et al. SUMOylation of Myc-family proteins. PLoS One 9:e91072 (2014). WB ; Human . PubMed: 24608896
- Weisshaar SR et al. Arsenic trioxide stimulates SUMO-2/3 modification leading to RNF4-dependent proteolytic targeting of PML. FEBS Lett 582:3174-8 (2008). PubMed: 18708055