Product nameAnti-Sumo 2 + Sumo 3 antibody [EPR300(2)]
See all Sumo 2 + Sumo 3 primary antibodies
DescriptionRabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Sumo 2 + Sumo 3 aa 1-100. The exact sequence is proprietary.
- Paraffin-embedded Human ovarian tissue HeLa and Jurkat cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
RelevanceSUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Cellular localizationCytoplasmic (SUMO3) and Nuclear (SUMO2)
- HSMT3 antibody
- MGC117191 antibody
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All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR300(2)] (ab109196) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa
ab109196, at 1/100, staining Sumo 2+3 in paraffin-embedded Human ovarian tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109196 has not yet been referenced specifically in any publications.